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ORIGINAL ARTICLE
Year : 2018  |  Volume : 4  |  Issue : 1  |  Page : 21-28

Evaluation of the efficacy of topical ofloxacin, pefloxacin, lomefloxacin, sparfloxacin in the experimental model of corneal ulcer


1 Department of Ocular Pharmacology and Pharmacy, Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi-110029, India
2 Department of Pharmacology, Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi-110029, India
3 Department of Medical Ophthalmology, Retina and Uvea, Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi-110029, India
4 Department of Pediatric Ophthalmology and Oculoplasty, Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi-110029, India

Correspondence Address:
Nihar Ranjan Biswas
Director & Vice-Chancellor, Indira Gandhi Institute of Medical Sciences, Sheikhpura, Patna-800014, Bihar; Department of Pharmacology, All India Institute of Medical Sciences, New Delhi- 110029; Director & Vice-Chancellor, Indira Gandhi Institute of Medical Sciences, Sheikhpura, Patna-800014, Bihar
India
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Source of Support: None, Conflict of Interest: None


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Objective: This study was designed to evaluate the efficacy of topical ofloxacin, pefloxacin, lomefloxacin, sparfloxacin in the experimental model of corneal ulcer. Methods: Twenty New Zealand albino rabbits of either sex weighing 1.5-2 kg body weight were used for the corneal ulcer efficacy study, Staphylococcus aureus was cultured in 1% peptone water by incubating it at 37? c for 48 hrs. The colony forming unit (CFU) was estimated by using standard McFarland’s tube. Sterile Saline (0.05 ml) containing Staph. Aureus 5x 106 CFU per ml was used for the induction of corneal ulcer. About 10-15μl of the incoculum was injected intrastromally into the peripheral cornea of rabbits using a 1 ml tuberculin syringe fitted with 30G needle. After delivery of inoculums, the epithelial layer was carefully removed by scratching with scalpel blade just above the inoculums delivery site. Before induction of corneal ulcer, all the rabbits were anaesthetized. The antimicrobial therapy was initiated after 18 hrs of the inoculation. 50 μl of formulation were instilled four times a day. Treatment was given up to 4 weeks in each group. Results: After 14 days of treatment with lomefloxacin and sparfloxacin a significant reduction in ulcer size was recorded in the animals. Where as the same was achieved in 7 days after topical ofloxacin treatment. Based on this observation, the efficacy of the tested formulations can be graded in this following order i.e pefloxacin = nanoparticulated sparfloxacin> sparfloxacin> lomefloxacin> ofloxacin. While comparing the percentage healing that occurred after two weeks of therapy, (using the ulcer size) pefloxacin (0.3%) showed 100% healing. Conclusion: Efficacy wise pefloxacin was found to be superior as compared to other fluoroquinolones studied.


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