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ORIGINAL ARTICLE
Year : 2019  |  Volume : 5  |  Issue : 2  |  Page : 126-130

Role of Xpert MTB/RIF assay (Gene Xpert) in diagnosing tuberculosis in elderly patients - A hospital based study


1 Junior Resident, Dept. of Microbiology, IGIMS, Patna, Bihar, India
2 Professor, Dept. of Microbiology, IGIMS, Patna, Bihar, India
3 Associate Professor, Dept. of Microbiology, IGIMS, Patna, Bihar, India
4 Senior Resident, Dept. of Microbiology, IGIMS, Patna, Bihar, India
5 Additional Professor, Dept. of Microbiology, IGIMS, Patna, Bihar, India
6 Professor & Head, Dept. of Microbiology, IGIMS, Patna, Bihar, India

Correspondence Address:
Rakesh Kumar
Associate Professor, Dept. of Microbiology, IGIMS, Patna
India
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Source of Support: None, Conflict of Interest: None


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Background: Tuberculosis is a major public health problem throughout the world. Tuberculosis has long been known as a disease of young people but many elderly (? 60 years of age) are now falling ill with TB. Several factors associated with aging result in delayed diagnosis and initiation of treatment. Aim of the Study:
  1. To detect the prevalence of tuberculosis (TB) and drug resistant TB (DR-TB) among elderly.
  2. To compare the role of molecular methods with conventional tests in diagnosis of tuberculosis in elderly patients.
Materials and Methods: Clinical samples received from patients were subjected to-
  1. Smear microscopy using Zeihl Neelsen (ZN) & Fluorescent staining
  2. Xpert MTB/RIF assay (GeneXpert test)
Results: Acid fast bacilli (AFB) were detected in 17.80% (73/410) of samples. Elderly contributed to 11.40% (73/640) of total TB cases. Gene Xpert detected M tb in 34 smear negative samples also which constituted 46.57% (34/73) of total positive. AFB detection by Gene Xpert test was the highest followed by Fluorescent & ZN staining. Majority of the samples were sensitive to rifampicin. Conclusion: Elderly TB patients have high smear negativity. Gene Xpert test allows early detection of smear negative and drug resistant cases. It may be offered as the first choice investigating modality. Early diagnosis and initiation of specific anti tubercular therapy will have a major impact in reducing the disease burden and achieving our goal of “TB Free India”.


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